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CCNY/ASRC FACILITIES


High-field NMR Resources

The CUNY ASRC Nuclear Magnetic Resonance Facility features three state-of-the-art Bruker AVANCE III HD NMR spectrometers operating at 600, 700, and 800 MHz suitable for the full complement of biomolecular NMR studies. All three spectrometers are equipped with either cryogenically-cooled or room temperature probes offering a wide range of 1H, 13C, 15N, 19F, 31P, and 2H NMR capabilities.

Equipped with refrigerated sample changers and remote operation capabilities, each of these instruments is able to independently run through 24 (SampleCase) or 600 (SampleJet) samples with limited user involvement.

Additional resources include (i) two laser systems dedicated for studies of photoreceptors or other photochemical systems, ii) a high-pressure system for acquiring NMR data at pressures up to 2500 bar, and iii) 1.6-mm HXY magic-angle-spinning (MAS) probe for solid-state NMR experiments, capable of sample MAS rates of up to 40 kHz, for use with the 600 MHz spectrometer.


Other Facilities

Facilities are available for theexpression, production, purification and biophysical characterization of isotope-labeled proteins suitable for NMR studies.
Computational facilities for MD simulations and highly parallel structure calculation protocols are also available.
MS facilities for the characterization and analysis of biomolecular samples.
Electron microscopy facilities.

More details may be found at: http://structbio.asrc.cuny.edu/facilities/

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NYSBC FACILITIES



The New York Structural Biology Center (NYSBC), a consortium of 10 New York area universities and research organizations, is situated on the City College campus. It is the largest facility of its kind in the United States.

High-field NMR Resources

500 MHz (1), 600 MHz (1), 700 MHz (1), 750 MHz (wide-bore, solids/liquids capable), 800 MHz (3) and 900 MHz (2). All spectrometers equipped with cryoprobes with z-gradients. All Bruker Avance series spectrometers.

X-Ray Crystallography

The NYSBC maintains the X4A beamline at the Brookhaven National Labs (4-20 KeV).

Electron Microscopy

Set up for electron crystallography, single-particle analysis and electron tomography. 120 kV (1, JEOL 1230, FEG), 200 kV (1, Tecnai TF20, LaB6; 1 JEOL 2100F FEG) and 300 kV (JEOL 3200FSC FEG). In addition all facilities for sample preparation and screening are available.