High-field NMR Resources
The CUNY ASRC Nuclear Magnetic Resonance Facility features three state-of-the-art Bruker AVANCE III HD NMR spectrometers operating at 600, 700, and 800 MHz suitable for the full complement of biomolecular NMR studies. All three spectrometers are equipped with either cryogenically-cooled or room temperature probes offering a wide range of 1H, 13C, 15N, 19F, 31P, and 2H NMR capabilities.
Equipped with refrigerated sample changers and remote operation capabilities, each of these instruments is able to independently run through 24 (SampleCase) or 600 (SampleJet) samples with limited user involvement.
Additional resources include (i) two laser systems dedicated for studies of photoreceptors or other photochemical systems, ii) a high-pressure system for acquiring NMR data at pressures up to 2500 bar, and iii) 1.6-mm HXY magic-angle-spinning (MAS) probe for solid-state NMR experiments, capable of sample MAS rates of up to 40 kHz, for use with the 600 MHz spectrometer.
Facilities are available for theexpression, production, purification and biophysical characterization of isotope-labeled proteins suitable for NMR studies.
Computational facilities for MD simulations and highly parallel structure calculation protocols are also available.
MS facilities for the characterization and analysis of biomolecular samples.
Electron microscopy facilities.
More details may be found at: http://structbio.asrc.cuny.edu/facilities/