We can no longer provide PDF copies of our manuscripts from this website due to copyright issues. Click on the logo on the left of the entry for the abstract (PubMed links used where available). Access to full-text versions may require journal subscription.

2024

Post PicPiserchio A, Dalby KN and Ghose R 2024. Revealing eEF-2 kinase: recent structural insights into function. Trends Biochem. Sci. 49, 169-182.

2023

Post PicPiserchio A, Isiorho EA, Dalby KN and Ghose R 2023. Structure of the complex between calmodulin and a functional construct of eukaryotic elongation factor 2 kinase bound to an ATP-competitive inhibitor. J. Biol. Chem. 299, 104813. PDB: 8GM4, 8GM5
Post PicPiserchio A, Long KJ, Browning LS, Bohanon, AL, Isiorho EA, Dalby KN and Ghose R 2023. ADP enhances the allosteric activation of eukaryotic elongation factor 2 kinase by calmodulin. Proc. Natl. Acad. Sci. USA 120, e2300902120. PDB: 8FO6, 8FNY
Post PicHajredini F, Alphonse S, and Ghose R 2023. BY-kinases: Protein tyrosine kinases like no other J. Biol. Chem. 299, 102737.

2022

Post PicPiserchio A, Isiroho EA, Long K, Bohanon AL, Kumar EA, Will N, Jeruzalmi D, Dalby KN, and Ghose R 2022. Structural Basis for the Calmodulin-mediated Activation of Eukaryotic Elongation Factor 2 Kinase. Science Adv. 8, eabo2039. PDB: 7SHQ
Post PicAlphonse S, Djemil I, Piserchio A and Ghose R 2022. Structural Basis for the Recognition of the Bacterial Tyrosine Kinase, Wzc by its Cognate Tyrosine Phosphatase, Wzb. Proc. Natl. Acad. Sci. USA 119, e2201800119.

2021

Post PicHajredini F and Ghose R 2021. A conserved structural role for the Walker-A lysine in P-Loop containing kinases. Front. Mol. Biosci. 8, 747206.
Post PicHajredini F and Ghose R 2021. An ATPase with a twist: A unique mechanism underlies the activity of the bacterial tyrosine kinase, Wzc. Science Adv. 7, eabj5836.
Post PicPiserchio A, Long K, Lee K, Kumar EA, Abzalimov R, Dalby KN and Ghose R 2021. Structural dynamics of the complex of calmodulin with a minimal functional construct of eukaryotic elongation factor 2 kinase and the role of Thr348 autophosphorylation. Prot. Sci. 30, 1221-1234.
HIGHLIGHTED ARTICLE
Post PicAlphonse S, Banerjee A, Dantuluri S, Shuman S and Ghose R 2021. NMR solution structures of Runella slithyformis RNA 2'-phosphotransferase Tpt1 provide insights into NAD+ binding and specificity. Nucl. Acids Res. 49, 9607-9624. PDB: 7KW8,7KW9
COVER FEATURE

2020

Post PicHajredini F, Piserchio A and Ghose R 2020. Long-range dynamic correlations regulate the catalytic activity of the bacterial tyrosine kinase Wzc. Science Adv. 6, eabd3718.
Post PicStenzoski NE, Zou J, Piserchio A, Ghose R, Holehouse AS and Raleigh DP 2020. The Cold-Unfolded State is Expanded but Contains Long-and Medium-range Contacts and Is Poorly Described by Homopolymer Models. Biochemistry 59, 3290-3299.
Post PicGhose R 2020. Keep a lid on it: A troika in kinase allostery. J. Biol. Chem. 295, 8492-8493.

2019

Post PicKaoud T, Johnson W, Ebelt N, Piserchio A, Zamora-Olivares D, Ravenstein S, Pridgen J, Edupuganti R, Sammons R, Cano M, Warthaka M, Harger M, Tavares C, Park J, Radwan M, Ren P, Anslyn E, Tsai K, Ghose R and Dalby KN 2019. Modulating multi-functional ERK complexes by covalent targeting of a recruitment site in vivo. Nature Commun. 10, 5232.
Post PicLee K, Kumar EA, Dalby KN and Ghose R 2019. The Role of Calcium in the Interaction between Calmodulin and a Minimal Functional Construct of Eukaryotic Elongation Factor 2 Kinase. Prot. Sci. 28, 2089-2098.
Post PicPiserchio A, Will N, Giles DH, Hajredini F, Dalby KN and Ghose R 2019. Solution Structure of the Carboxy-terminal Tandem Repeat Domain of Eukaryotic Elongation Factor 2 Kinase and its Role in Substrate Recognition. J. Mol. Biol. 431, 2700-2717. PDB:6NX4
Post PicSammons R, Ghose R, Tsai K and Dalby KN. 2019. Targeting ERK beyond the boundaries of the kinase active site in melanoma. Mol. Carcinogen. 58, 1551-1570.
COVER FEATURE
Post PicSammons RM, Perry NA, Li Y, Cho EJ, Piserchio A, Zamora-Olivares DP, Ghose R, Kaoud TS, Debevec G, Bartholomeusz C, Gurevich VV, Iverson TM, Guilianotti M, Houghten RA and Dalby KN. 2019. A Novel Class of Common Docking Inhibitors that Prevent ERK2 Activation and Substrate Phosphorylation. ACS Chem. Biol. 14, 1183-1194.
Post PicGhose R 2019. Nature of the Pre-Chemistry Ensemble in Mitogen-Activated Protein Kinases. J. Mol. Biol. 431, 145-157.

2018

  • Post PicAlphonse S and Ghose R 2018. Methyl NMR Spectroscopy: Measurement of Dynamics in Viral RNA-directed RNA Polymerases. Methods, 148, 100-114.
    Post Pic    Will N, Lee K, Hajredini F, Giles DH, Abzalimov RR, Clarkson M, Dalby KN and Ghose R 2018. Structural Dynamics of the Activation of Elongation Factor 2 Kinase by Ca2+-Calmodulin. J. Mol. Biol. 430, 2802-2821.
    Post PicAlphonse S, Itin B, Khayat R and Ghose R 2018.Sequential Protein Expression and Capsid Assembly in Cell: Toward the Study of Multiprotein Viral Capsids Using Solid-State Nuclear Magnetic Resonance Techniques. Biochemistry, 57, 1568-1571.

Protein NMR - Methods and Protocols (ISBN 978-1-4939-7386-6).

2017

  • Post PicPiserchio A, Warthaka M, Kaoud TS, Callaway K, Dalby KN and Ghose R 2017. Local destabilization, rigid-body and fuzzy docking facilitate the phosphorylation of Ets-1 by ERK2. Proc. Natl. Acad. Sci. USA. 114, E6287-E6296.
    Post PicReed BR, Yakovleva L, Shuman S and Ghose R 2017.Characterization of DNA binding by the isolated N-terminal domain of vaccinia virus DNA topoisomerase IB. Biochemistry. 56, 3307-3317.
    Post PicAlphonse S and Ghose R 2017. Cystoviral RNA-directed RNA Polymerases: Regulation of RNA Synthesis on Multiple Time and Length Scales. Virus Res. 234, 135-152.
    Post Pic Ghose R 2017. Protein Structure and Interactions from Nuclear Magnetic Resonance Spectroscopy.In Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd.:Chichester.
  • Post PicTavares CDJ, Giles DH, Stancu BG, Chitjian CA, Ferguson SB, Wellman RM, Kaoud TS, Ghose R and Dalby KN 2017. Signal integration at elongation factor 2 kinase; the roles of calcium, calmodulin and Ser-500 phosphorylation. J. Biol. Chem. 292, 2032-2045.

2016

  • Post PicWill N, Piserchio A, Snyder I, Ferguson SB, Giles DH, Dalby KN and Ghose R 2016. Structure of the C-terminal Helical Repeat Domain of Eukaryotic Elongation Factor 2 Kinase. Biochemistry, 55, 5377-5386. PDB:KS5
    Post PicLee K, Alphonse S, Piserchio A, Tavares CDJ, Giles DH, Wellmann RM, Dalby KN and Ghose R 2016. Structural Basis for the Recognition of Eukaryotic Elongation Factor 2 Kinase by Calmodulin. Structure, 24, 1441-1451. PDB:5J8H
    PREVIEW BY VEGLIA & LI

2015

  • Post PicAlphonse S, Bhattacharya S, Wang H and Ghose R 2015. Methyl Relaxation Measurements Reveal Patterns of Fast Dynamics in a Viral RNA-Directed RNAPolymerase. Biochemistry, 54, 5828.
    Post PicPiserchio A, Ramakrishan V, Wang H, Kaoud TS, Arshava B, Dutta K, Dalby K and Ghose R 2015. Structural and Dynamic Features of F-recruitment Site Driven Substrate Phosphorylation by ERK2. Sci. Rep. 5, 11127.

2014

  • Post PicAlphonse S, Arnold JJ, Bhattacharya S, Wang H, Kloss B, Cameron CE and Ghose R 2014.Cystoviral Polymerase Complex Protein P7 Uses its Acidic C-terminal Tail to Regulate the RNA-directed RNA Polymerase P2. J. Mol. Biol. 426, 2580-2593.
    Post Pic    Temel DB, Dutta K and Ghose R 2014. Sequence-specific 1H, 13C and 15N assignments of the catalytic domain of the Escherichia coli tyrosine kinase, Wzc. Biomolec. NMR Assignments. 8, 37-41.

2013

  • Post PicRen Z, Franklin MC and Ghose R 2013. Structure of the RNA-directed RNA polymerase from the cystovirus Φ12. Proteins: Struct. Func. Bioinform. 81, 1479-1484. PDB:4IEG; 4GZK
  • Post PicTemel DB, Dutta K, Alphonse S, Nourikyan J, Grangegasse C and Ghose R. 2013. Regulatory interactions between a bacterial tyrosine kinase and its cognate phosphatase. J. Biol. Chem. 288, 15212-15228.
  • Post PicGhose R 2013. A measured approach: determining the PLCγ1 docking site on Itk using a biochemical ruler. J. Mol. Biol. 425, 679-682.
  • Ghose R. 2013 Detection of Slow Dynamics Using Multiple-Quantum NMR Spectroscopy. In: Encyclopedia of Biophysics, Ed. Gordon Roberts, Springer Reference, Springer-Verlag, Berlin, Heidelberg.

2012

Post PicPiserchio A, Francis D, Koveal D, Dalby KN, Page R, Peti W and Ghose R 2012. Docking Interactions of Hematopoietic Tyrosine Phosphatase (HePTP) with the MAP Kinases ERK2 and p38α. Biochemistry, 51, 8047-8049.
Post PicPiserchio A, Cowburn D and Ghose R 2012. Expression and Purification of Src-family KInases for Solution NMR Studies. Meth. Mol. Biol. 831, 111-131.
Post PicPiserchio A, Dalby K and Ghose R 2012. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase - ERK2 as an Illustrative Example. Meth. Mol. Biol. 831, 359-368.
Post PicNatarajan A, Dutta K, Temel DB, Nair PA, Shuman S and Ghose R 2012. Solution structure and DNA-binding properties of the phosphoesterase domain of DNA ligase D. Nucl. Acids Res. 40, 2076-2088. PDB:2LJ6

2011

Post PicPiserchio A, Warthaka M, Devkota A, Kaoud TS, Lee S, Abramczyk O, Ren P, Dalby KN and Ghose R 2011. Solution NMR Insights into Docking Interactions Involving Inactive ERK2. Biochemistry 50, 3660-3772.
Post PicLee S, Warthaka M, Yan C, Kaoud TS, Piserchio A, Ghose R, Ren P and Dalby KN 2011. A Model of a MAPK-Substrate Complex in Active Conformation: A Computational and Experimental Approach. PLOS One 6, e15894.
Post PicDutta K, Natarajan A, Nair P, Shuman S and Ghose R 2011. Sequence-specific 1H, 13C and 15N assignments of the phosphoesterase (PE) domain of Pseudomonas aeruginosa DNA ligase D (LigD). Biomolec. NMR Asgn. 5, 151-155.
Post PicRen Z and Ghose R 2011. Slow Conformational Dynamics in the Cystoviral RNA-directed RNA Polymerase P2 – Influence of Substrate Nucleotides and Template RNA. Biochemistry 50, 1875-1884.

2010

Post PicFerrage F, Riechel A, Bhattacharya S, Cowburn D and Ghose R 2010. On the measurement of 15N-{1H} nuclear Overhauser effects 2. Effects of the saturation scheme and water signal suppression. J. Magn. Reson. 207, 294-303.
Post PicRen Z, Wang H and Ghose R 2010. Dynamics on multiple timescales in the RNA-directed RNA polymerase from the cystovirus Φ6. Nucleic Acids Res. 38, 5105-5118.
Post PicNicholas MP, Eryilmaz E, Ferrage F, Cowburn D and Ghose R 2010. Nuclear Spin Relaxation in isotropic and anisotropic media. Prog. NMR Spectroscopy 57, 111-158.
Post Pic Greenbaum NL and Ghose R 2010. Nuclear magnetic resonance (NMR) spectroscopy:Structure determination of proteins and nucleic acids. In Encyclopedia of Life Sciences (ELS). John Wiley & Sons, Ltd.:Chichester.
Post PicPiserchio A, Nair P, Shuman S and Ghose R 2010. Solution NMR Studies of Chlorella Virus DNA Ligase-Adenylate. J. Mol. Biol. 395, 291-308.

2009

Post PicPiserchio A, Ghose R and Cowburn D 2009. Optimized Bacterial Expression and Purification of the c-Src Catalytic Domain for Solution NMR Studies. J. Biomol. NMR 44, 87-93.
Post PicFerrage F, Cowburn D and Ghose R 2009. Accurate Sampling of High-Frequency Motions in Proteins by Steady-State 15N-{1H} Nuclear Overhauser Effect Measurements in the Presence of Cross-Correlated Relaxation. J. Am. Chem. Soc. 131, 6048-6049.
Post PicPiserchio A, Nair P, Shuman S and Ghose R 2009. Sequence-specific 1HN, 13C and 15N backbone resonance assignments of the 34 kDa paramecium bursaria chlorella virus 1 (PBCV1) DNA ligase. Biomol. NMR Assign. 3, 77-80.

2008

Post PicEryilmaz E, Benach J, Su M, Seetharaman J, Dutta K, Wei H, Gottlieb P, Hunt JF and Ghose R 2008. Structure and Dynamics of the P7 Protein from the Bacteriophage Φ12. J. Mol. Biol. 382, 402-422. PDB: 2Q82
Post PicFerrage F, Piserchio A, Cowburn D and Ghose R 2008. On the Measurement of 15N-{1H} Nuclear Overhauser Effects. J. Magn. Reson. 192, 302-313.

2007

Post PicDel Rio A, Dutta K, Chavez J, Ubarretxena-Belandia I and Ghose R 2007. Solution Structure and Dynamics of the N-terminal Cytosolic Domain of Rhomboid Intramembrane Protease from Pseudomonas aeruginosa: Insights into a Functional Role in Intramembrane Proteolysis. J. Mol. Biol. 365, 109-122. PDB: 2GQC

2006

Post PicNatarjan A, Ghose R and Hill JM. 2006 Structure and Dynamics of ASC2, a Pyrin Domain-only Protein that Regulates Inflammatory Signalling. J. Biol. Chem. 281, 31863-31875. PDB: 2HM2
Post PicJi H, Shekhtman A, Ghose R, McDonnell J and Cowburn D 2006. NMR determination that the BH3 motif of pro-apoptotic BID possesses extended specificity bound to BCL-XL. Magn. Reson. Chem. 44, S101-107.
Post PicPujato M, Navarro A, Versace R, Mancusso R, Ghose R and Tasayco ML 2006. Do Carboxylate Sidechains and Backbone Amides of Asp and Glu Exhibit Similar pH-Dependence in Unfolded Proteins? BBA Proteins and Proteomics, 1764, 1227-1233.
Post PicDel Rio A, Anand A and Ghose R 2006. Detection of Correlated Dynamics on Multiple Timescales by Measurement of the Differential Relaxation of Zero- and Double-Quantum Coherences Involving Sidechain Methyl Groups in Proteins. J. Magn. Reson. 180, 1-17.

2005

Post PicPradhan P, Ghose R and Green ME 2005. Voltage Gating and Anions, especially Phosphate: A Model System. BBA Biomembranes 1717, 97-103.

2004

Post PicDutta K, Shi HH, Cruz-Chu ER, Kami K and Ghose R 2004 Dynamic Influences on a High-affinity, High-specificity Interaction Involving the C-terminal SH3 Domain of p67phox Biochemistry 43, 8094-8106
Post PicMajumdar A and Ghose R 2004. Probing slow backbone dynamics in proteins using TROSY-based experiments to detect cross-correlated time-modulation of isotropic chemical shifts. J. Biomol. NMR 28, 213-227.

1993-2003

Post PicShekhtman A, Ghose R, Goger MJ and Cowburn D 2002. NMR structure determination and investigation using a reduced proton (REDPRO) labeling strategy for proteins. FEBS Lett. 524, 177-182
Post PicShekhtman A, Ghose R, Cole PA and Cowburn D 2001. Novel mechanism of regulation of the non-receptor protein tyrosine kinase Csk : Insights from NMR mapping studies and site-directed mutagenesis. J. Mol. Biol. 314, 129-138.
COVER FEATURE
Post PicGhose R, Shekhtman A, Goger MJ, Ji H and Cowburn D 2001. A novel, specific interaction involving the Csk SH3 domain and its natural ligand. Nature Struct. Biol. 8, 998-1004. PDB: 1JEG
Post PicGhose R, Fushman D and Cowburn D 2001. Determination of the Rotational Diffusion Tensor of Macromolecules in Solution from NMR Relaxation Data with a Combination of Exact and Approximate Methods. J. Magn. Reson. 149, 204-217.
Post PicFushman D, Ghose R and Cowburn D 2000. The Effect of Finite Sampling on the Determination of Orientational Properties: A Theoretical Treatment with Application to Interatomic Vectors in Proteins. J. Am. Chem. Soc. 122, 10640-10649.
Post Pic Ghose R. 2000. Average Liouvillian Theory in Nuclear Magnetic Resonance - Principles, Properties and Applications. Concepts Magn. Reson. 12, 152-172.
Post PicChiarparin E, Pelupessy P, Ghose R and Bodenhausen G. 2000. Relative Orientation of CαHα Bond Vectors of Successive Residues in Proteins through Cross-correlated Relaxation in NMR. J. Am. Chem. Soc. 122, 1758-1761.
Post PicPelupessy P, Chiarparin E, Ghose R and Bodenhausen G. 1999. Simultaneous Determination of ψ and φ Angles in Proteins from Measurements of Cross-correlated Relaxation Effects. J. Biomol. NMR 14, 277-280.
Post PicHuang K, Ghose R, Flanagan JM and Prestegard JH. 1999. Backbone Dynamics of the N-terminal Domain in E. coli DnaJ determined by 15N and 13CO Relaxation Measurements. Biochemistry 38,10567-10577.
Post PicChiarparin E, Pelupessy P, Ghose R and Bodenhausen G. 1999 Relaxation of Two-Spin Coherence due to Cross-Correlated Fluctuations of Dipole-Dipole Couplings and Anisotropic Shifts in NMR of 15N, 13C-labeled Biomolecules. J. Am. Chem. Soc. 121,6876-6883.
Post PicCutting B, Ghose R and Bodenhausen, G 1999. Tilt Angle Dependence of Cross Relaxation in Off-Resonance ROESY. J. Magn. Reson. 138, 326-329.
Post PicPelupessy P, Chiarparin E, Ghose R and Bodenhausen G 1999. Efficient Determination of Angles Subtended by Ca-Ha and N-H(N) Vectors in Proteins Via Dipole-Dipole Cross-correlation. J. Biomol. NMR 13, 375-380.
Post Pic Ghose R, Eykyn, T and Bodenhausen G 1999. Average Liouvillian Theory Revisited : Cross Correlated Relaxation between Chemical Shift Anisotropy and Dipolar Couplings in the Rotating Frame in Nuclear Magnetic Resonance. Mol. Phys. 96, 1281-1288.
Post PicEykyn TR, Ghose R and Bodenhausen G 1999. Offset Profiles of Selective Pulses in Isotopically Labeled Macromolecules. J. Magn. Reson. 136, 211-213.
Post PicGhose R, Huang K and Prestegard JH. 1998. Measurement of Cross Correlation between Dipolar Coupling and Chemical Shift Anisotropy in the Spin Relaxation of 13C, 15N-Labeled Proteins. J. Magn. Reson. 135, 487-499.
Post PicGhose R and Prestegard JH. 1998. Improved Estimation of CSA-Dipolar Coupling Cross-correlation Rates from Laboratory-frame Relaxation Experiments. J. Magn. Reson. 134, 308-314.
Post PicGhose R and Prestegard JH. 1997. Electron Spin - Nuclear Spin Cross-correlation Effects on Multiplet Splittings in Paramagnetic Proteins. J. Magn. Reson. 128, 138-143.
Post PicGhose R, Anderson CA and Prestegard JH. 1997. Origin of the Correlation Time Dependence of Coherence Transfer Distortions in Rotating Frame Cross-relaxation Spectra. J. Magn. Reson. 128, 207-216.
Post PicGhose R, Geiger O and Prestegard JH. 1996. NMR Investigations of the Structural Properties of the Nodulation Protein, NodF, from Rhizobium leguminosarum and its Homology with the Escherichia coli Acyl Carrier Protein. FEBS Lett. 388, 66-72.
Post PicSalvatore BA, Ghose R and Prestegard JH. 1996. NMR Studies of a 13C, 15N-Labeled GM4 Lactam Glycolipid at an Oriented Model-Membrane Interface. J. Am. Chem. Soc. 118, 4001-4008.
Post PicGhose R. , Marino JP, Wiberg KB and Prestegard JH. 1994. Dependence of 13C Chemical Shifts on Glycosidic Torsional Angles in Ribonucleic Acids. J. Am. Chem. Soc. 116, 8827-8828.